from the bench of sabominator

the view for about 20 minutes when i should have been eating lunch:

-rinse and develop membrane 038_1

-incubate membrane 037_3 in multiblotter for primaries, 8 different antibodies

-permeabilize membrane 037_4 in PBS

-nutate blocker for membrane 037_4

-watch the 5 gels running in 3 gel tanks, attempt to finish run simultaneousy; 1 tank is leaking, so use 2 power supplies at different voltages (at opposite ends of the lab)

-set up tanks for transferring the four 2D gels

-equilibrate membranes, filter paper and fiber pads in transfer buffer

-oops, make more transfer buffer

-oops, make more TBS-T for washing membranes

-oops, make more fixer for remaining gel to be silver stained

-make more buffer for FPLC mobile phase

-order another chemiluminescence kit, more film

-weigh out reagents for tomorrow's 043_1 crosslinking/size exclusion chromotography experiment

-prepare the lab for safety inspection tomorrow

-"hey sabrina, are you coming to your boss' seminar in 10 minutes?"

-"no."

*brief reprieve: 7 minute lunch, chinese truck*

wash; rinse; repeat for the next 3 hours...

boss comes by..."hey, how are things going?"

"busy."

"oh. well have you gotten anything done?"

"um, yes."

"on the poster i mean."

"err...i haven't really had time...that collecting data stuff is a real killer."

"oh. ok. well, have a draft by next wednesday."

sure dude, sure.

T MINUS 14 WORK DAYS AND COUNTING UNTIL DEPARTURE FOR NEUROSCIENCE.